Signal Enhancer PO-5 is a lipid polymer based on phosphorylcholine groups. Its molecular structure mimics the hydrophilic portion of biological cell membranes (phospholipid heads), endowing it with excellent biocompatibility and multifunctionality. It is widely used in immunoassay platforms (such as turbidimetric assays, colloidal gold, and latex particle-based tests) to enhance detection sensitivity and accuracy by improving signal stability and anti-interference capability.

Mechanism of Action

·High Biocompatibility: The phosphorylcholine groups resemble biological membrane structures, reducing non-specific adsorption and minimizing background noise.

·High Hydrophilicity: Forms a hydration layer to stabilize colloidal particles or latex microspheres, preventing aggregation.

·High Lubricity: Reduces intermolecular friction, optimizing lateral flow or hydrodynamic performance (e.g., uniform migration in lateral flow strips).

·Anticoagulant Property: Inhibits platelet adhesion, making it suitable for direct detection of blood samples (e.g., whole blood rapid diagnostics).

·Antioxidant Protection: Chelates metal ions or scavenges free radicals, protecting active components (e.g., antibodies, enzymes) from oxidative inactivation.

Platform Application and Function

Turbidimetric Assay: Added to reagent R1 to enhance the turbidity signal of antigen-antibody complexes and improve the detection linear range.

Colloidal Gold/Latex Lateral Flow: Pre-mixed in the sample diluent or conjugate pad to reduce marker aggregation and enhance the intensity and uniformity of the colorimetric signal.

Other Immunoassays: Serves as a universal stabilizer in assays such as ELISA and chemiluminescence to reduce matrix effects.

Operation

Dosage: Dilute the signal enhancer PO-5 by 10-30 times. For example: 95 ml sample diluent + 5 ml PO-5 (equivalent to a 20-fold dilution). If using the product for a specific assay for the first time, a gradient test is recommended.

Lateral Flow: Preferably add it to the sample diluent; alternatively, add it to the conjugate pad.

Turbidimetric Assay: Add it to reagent R1.