S12 Surfactant

AccuSift
IGEPAL CA-210
SKU Name Unit Price Delivery QTY
BLDS12 S12 Surfactant 100G $83.3 10days
BLDS12 S12 Surfactant 500G $333.4 10days

Non-ionic surfactant, exhibiting excellent dispersion, diffusion, wetting, and emulsifying properties in water, with film-forming capability. Resistant to acids and alkalis.

Mechanism of Action

Emulsification and Dispersion

As a non-ionic surfactant, it reduces interfacial tension between aqueous and organic phases (e.g., lipids, oil-soluble substrates), forming stable oil-in-water (O/W) emulsions to prevent component stratification or precipitation.
Film-forming ability: Creates a hydrophobic protective film on the inner walls of microplates, reaction tubes, or sample surfaces, prolonging the stability of active reagent components.

Acid and Alkali Resistance

Maintains activity under strongly acidic (pH < 2) or alkaline (pH > 12) conditions, making it suitable for detection steps requiring extreme pH environments (e.g., viral lysis buffer preparation).

Wetting and Penetration Enhancement

Improves reagent spreading on hydrophobic materials (e.g., plastic microplates, test strips), enhancing reaction homogeneity.

Application Scenarios

Immunoassays

Cell lysis buffer: Gently disrupts cell membranes to release antigens while avoiding protein denaturation (e.g., viral antigen extraction in ELISA).

Blocking buffer/diluent: Reduces non-specific adsorption and background signals.

Molecular Diagnostics

Nucleic acid extraction: Aids in disrupting cell membranes or viral envelopes to improve DNA/RNA release efficiency.

PCR pre-treatment: Eliminates lipid interference in samples, enhancing amplification specificity.

Biochemical Detection

Enzymatic reaction systems: Stabilizes hydrophobic substrates (e.g., lipid substrates in cholesterol oxidase assays).

Microfluidic chips: Acts as a lubricant to reduce fluid flow resistance and prevent bubble interference.

Point-of-Care Testing (POCT)

Test strip coating: Optimizes sample diffusion speed on nitrocellulose membranes, shortening detection time.



Operation

Conventional Concentration Range

Cell Lysis Buffer: 0.1% - 1% (compatibility with the target protein must be validated).

Blocking Buffer/Diluent: 0.05% - 0.5%.

Nucleic Acid Extraction: 0.5% - 2% (used in combination with Proteinase K).

Enzymatic Reaction Systems: 0.01% - 0.1% (avoid concentrations that inhibit enzyme activity).

Dissolution Method

Direct Dissolution: Stir to dissolve in water or buffer at room temperature. If necessary, heat to 40-50°C to accelerate dissolution.

Premixed Storage: It is recommended to prepare a 10%-20% stock solution. Avoid long-term storage to prevent stratification.

Precautions

Store sealed, protected from light, in a cool, dry place (recommended temperature: 15-25°C). Avoid high temperatures or direct light exposure to prevent degradation.

Stratification may occur during long-term storage; shake well before use.

Temperature Sensitivity: High temperatures (>60°C) may compromise emulsifying properties. Preparation at lower temperatures is recommended.

Oxidation Risk: Avoid direct mixing with strong oxidizing agents (e.g., high-concentration persulfates).

Plastic Containers: Prolonged contact may cause swelling of polystyrene (PS) or polycarbonate (PC) containers. Short-term use is advised, or switch to glass containers.

Protein Interference: Excessive concentrations may lead to adsorption of antibodies or antigens. Optimize concentration through preliminary experiments.

Compatibility Contraindications

Cationic Surfactants: May cause precipitation due to charge neutralization; avoid combining.

High-Salt Systems: May reduce emulsification efficiency; adjust concentration or add stabilizers if necessary.


References