Conventional Concentration Range•Cell Lysis Buffer: 0.1% - 1% (compatibility with the target protein must be validated).
•Blocking Buffer/Diluent: 0.05% - 0.5%.
•Nucleic Acid Extraction: 0.5% - 2% (used in combination with Proteinase K).
•Enzymatic Reaction Systems: 0.01% - 0.1% (avoid concentrations that inhibit enzyme activity).
•Direct Dissolution: Stir to dissolve in water or buffer at room temperature. If necessary, heat to 40-50°C to accelerate dissolution.
•Premixed Storage: It is recommended to prepare a 10%-20% stock solution. Avoid long-term storage to prevent stratification.
•Store sealed, protected from light, in a cool, dry place (recommended temperature: 15-25°C). Avoid high temperatures or direct light exposure to prevent degradation.
•Stratification may occur during long-term storage; shake well before use.
•Temperature Sensitivity: High temperatures (>60°C) may compromise emulsifying properties. Preparation at lower temperatures is recommended.
•Oxidation Risk: Avoid direct mixing with strong oxidizing agents (e.g., high-concentration persulfates).
•Plastic Containers: Prolonged contact may cause swelling of polystyrene (PS) or polycarbonate (PC) containers. Short-term use is advised, or switch to glass containers.
•Protein Interference: Excessive concentrations may lead to adsorption of antibodies or antigens. Optimize concentration through preliminary experiments.
Compatibility Contraindications
•Cationic Surfactants: May cause precipitation due to charge neutralization; avoid combining.
•High-Salt Systems: May reduce emulsification efficiency; adjust concentration or add stabilizers if necessary.