•TMB Stability Control:

•Terminates Oxidation Reaction: Aerosol OT forms a stable dark blue complex with TMB (3,3',5,5'-tetramethylbenzidine), preventing its further oxidation to the brown end product (quinoneimine form).

•Enhances Oxidized State Stability: The complex remains in the highest oxidation state (TMB⁺⁺), significantly prolonging the chromogenic duration of TMB and preventing signal decay.

•Improved Solubility and Compatibility:

•Enables the TMB-TMB complex to be compatible with both aqueous and organic solvents (e.g., toluene), broadening the applicability of detection methods (e.g., organic solvent extraction or biphasic reaction systems).

•ELISA (Enzyme-Linked Immunosorbent Assay): Stabilizes the TMB substrate, ensuring consistent and prolonged color development.

•Chemiluminescence Kits: Enhances luminescence efficiency and signal stability.

•POCT (Point-of-Care Testing) Devices: Maintains TMB activity in microplates or test strips, reducing inter-batch variability.

•DNA/RNA Amplification Reagents: Improves surface tension during nucleic acid extraction or hybridization, promoting reagent penetration.

•Cell Lysis Buffers: Serves as a mild, non-hemolytic surfactant to assist cell membrane disruption without hemoglobin release interference.

•ELISA Chromogenic Solution: 0.1%-0.3% (added after stopping the reaction)

•Chemiluminescence Substrate: 0.05%-0.2% (pre-mixed into the luminescence system)

•Microplate Pretreatment: 0.01%-0.05% (for surface wetting before coating)

•TMB-HCl chromogenic solutions (e.g., ELISA stop solution), chemiluminescence substrate systems, microplate pretreatment solutions.

•Biotin/avidin labeling systems: Enhances conjugation efficiency.

•Storage Conditions: Store sealed and protected from light. Avoid moisture and ethanol evaporation, which may cause gelation (if gelled, add 1%-2% ethanol to redissolve).

•Recommended Container Materials: Stainless steel, aluminum, or glass-lined containers (avoid long-term contact with plastic).

•pH Range: Use only under acidic/neutral conditions (pH ≤ 7). Decomposes and becomes ineffective in strongly alkaline environments.

•Temperature Adaptability: Freeze-thaw cycles do not affect activity, but ensure thorough mixing before use.

•Avoid direct mixing with strong oxidizing agents (e.g., high-concentration H₂O₂), as this may cause complex dissociation.

•When used in protein-containing systems (e.g., serum samples), validate whether it affects antibody binding activity.