S1 Surfactant

AccuSift
Ninate411
SKU Name Unit Price Delivery QTY
BLDS01 S1 Surfactant 100G $83.3 10days
BLDS01 S1 Surfactant 500G $334.2 10days
Ninate 411 is an anionic surfactant with strong detergency, high thermal stability, and acid resistance. It is suitable for diagnostic reagent systems requiring high-temperature processing or acidic environments. Its unique chemical stability and emulsifying capacity make it excel in sample lysis, latex particle stabilization, and complex matrix processing.

Mechanism of Action

Strong Detergency: Efficiently lyses cell membranes (e.g., red blood cells, bacteria) to release intracellular targets (e.g., nucleic acids, antigens), suitable for whole blood/tissue sample pretreatment.

Activity in Acidic Environments: Remains stable in low-pH buffers, compatible with catalytic reaction systems involving pepsin or acidic hydrolases (e.g., digestion buffer preparation).

High Thermal Stability: Withstands high-temperature sterilization (e.g., autoclaving or lyophilization processes), extending reagent shelf life.

Emulsification and Dispersion: Stabilizes latex/colloidal gold particles, preventing signal fluctuations caused by aggregation (e.g., immunoturbidimetric assays).

Anti-Protein Adsorption: Reduces nonspecific binding through electrostatic repulsion, lowering background interference (e.g., ELISA plate blocking).

Application Scenarios

Whole Blood Lysis Buffer: Added to lysis buffers to rapidly dissolve red blood cell membranes while preserving leukocyte or target protein integrity.

Latex-Enhanced Immunoturbidimetry: Combined with latex microspheres to enhance the turbidity signal of antigen-antibody complexes via electrostatic stabilization.

Enzyme Reaction System Stabilizer: Used in storage solutions for thermostable enzymes (e.g., Taq polymerase) to prevent enzyme aggregation and inactivation.

Solid Carrier Blocking Buffer: Combined with BSA to block nitrocellulose membranes or microplates, reducing nonspecific adsorption.

 

Operation

General Usage Range: 0.01%–0.5% (gradient testing is required to avoid excessive concentration causing protein denaturation or membrane damage).

References