AOS (Sodium Alpha-Olefin Sulfonate) is an anionic surfactant synthesized by sulfonation and neutralization of C14-16 olefins. It exhibits strong detergency, excellent wetting capability, high foam stability, and environmental friendliness (biodegradability >90%). Its mild chemical properties (low skin irritation) and enzyme compatibility make it suitable for in vitrodiagnostic reagents in sample pretreatment, colloidal particle stabilization, and reaction interface optimization.

•Strong Detergency and Wetting Ability: Efficiently lyses cell membranes or dissolves lipid/protein complexes to release target analytes (e.g., whole blood/tissue sample pretreatment).

•Enzyme Compatibility: Compatible with proteases, nucleases, and other enzymes, making it adaptable to enzyme-containing reaction systems (e.g., PCR lysis buffers, chemiluminescence wash solutions).

• Foam Stability: Facilitates homogeneous reactions by stabilizing foam structure (concentration must be controlled to avoid interference with optical detection).

•Biodegradability: Environmentally friendly, aligning with green chemistry principles and reducing the environmental impact of reagent waste.

•Formulation Compatibility: Can be combined with anionic/non-ionic surfactants (e.g., Tween 20) and chelating agents (e.g., EDTA) to accommodate complex reagent systems.

•Whole Blood/Tissue Lysis Buffer: Added to lysis buffers to rapidly dissolve cell membranes and release nucleic acids or antigens (e.g., PCR, ELISA sample preparation).

•Latex Particle Stabilizer: Pre-mixed in latex microparticle suspensions to prevent aggregation via electrostatic repulsion (e.g., immunoturbidimetric assays).

•Wash/Blocking Buffer: Used in ELISA or chemiluminescence wash solutions to gently remove unbound substances and reduce background interference.

•Hydrophobic Target Solubilizer: Solubilizes lipid-soluble molecules (e.g., cholesterol, membrane proteins) at a final concentration of 0.1%–0.3% to enhance detection sensitivity (e.g., lipid detection reagents).

•Microfluidic Chip Cleaner: Prepared as a low-concentration solution to remove protein residues from microchannels and ensure fluid uniformity.

•Concentration Control: Excessive use (>0.5%) may disrupt protein structures due to strong detergency or cause foam interference in optical detection.

•Compatibility Testing: Avoid direct mixing with cationic surfactants (e.g., CTAB) or high-concentration salts (e.g., ammonium sulfate), as precipitation may occur.

•When used with enzymes, validate the activity retention rate (e.g., final concentration ≤0.2%).

•Increased Detection Background: May result from excessively high AOS concentration or insufficient washing. It is recommended to reduce the concentration to 0.05%–0.1% and increase the number of wash cycles.

•Latex Particle Aggregation: May be caused by reaction with cationic components. Consider switching to a neutral/anionic buffer system (e.g., Tris-HCl).

•Foam Interference with Optical Signals: May result from excessive concentration or overly vigorous mixing. Reduce the concentration to below 0.1% or add a defoaming agent (e.g., Antifoam A).

•Reduced Enzyme Activity: May be due to surfactant interference with enzyme structure. Reduce the concentration to below 0.05% or consider replacing with Brij 35.