Specific interference is mainly caused by heterophilic antibodies (human anti-animal antibodies), which can lead to significant false positive or false negative results. It is reported that such interference can occur in 30-40% of patient samples. These heterophilic antibodies can be IgAs, IgEs, IgGs, IgMs, or Ig(n). They may exist as polymers or monomers and vary widely in concentration. However, they all directly target the specific antibodies in the detection system.Since most immunoassays use monoclonal mouse antibodies, human anti-mouse antibodies (HAMA) are the primary cause of assay interference.Interference elimination is a critical task in the development of immunoassay kits. Achieving higher sensitivity, improved precision, and more convenient operation requires effective interference elimination.The design of the assay significantly affects interference elimination. For example, IgG1 detection requires elimination of IgG1 interference, just as IgG2a detection requires elimination of IgG2a interference. HBR33poly is an IgG1 polymer blocker.HBR33poly can eliminate HAMA, rheumatoid factor (RF), and other heterophilic interferences that may cause false positive or false negative results.
WeChat
