This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Aliases CED4; APAF-1
Entrez GeneID 317
Swissprot O14727
WB Predicted band size 141.8kDa
Host/Isotype Mouse IgG2b
Species Reactivity Human
Immunogen Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.
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