This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Aliases :   CED4; APAF-1    

Entrez GeneID :   317    

Swissprot    O14727         

WB Predicted band size :   141.8kDa    

Host/Isotype :   Mouse IgG2b        

Species Reactivity :   Human    

Immunogen :   Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.    

IF/ICC    1/100 - 1/500    

FCM    1/200 - 1/400    

ELISA    1/10000