•High Water Solubility: Forms hydrogen bonds with water via polar hydroxyl groups in its molecular chain, significantly enhancing the solubility of lipid-soluble components (e.g., certain organic substrates, antibody labels) in aqueous phases.

•Prevention of Precipitation: Reduces system viscosity and inhibits component precipitation, maintaining the homogeneity of detection systems (e.g., stabilizing chemiluminescent substrates or nucleic acid probes).

•Friction Reduction: Acts as a lubricant in microfluidic chips and pipette tips, reducing fluid flow resistance.

•Antistatic Adsorption Prevention: Minimizes static charge accumulation on instrument surfaces, preventing loss of samples or reagents due to electrostatic adsorption.

•Reaction Rate Control: High concentrations of PEG 8000 can prolong reagent diffusion time, optimizing the dynamic balance of enzymatic reactions or molecular hybridization.

•Sustained-Release Carrier: Serves as a carrier for the sustained release of drugs or probes, extending detection signal duration.

•ELISA/Chemiluminescence: Used as a component in dilution or blocking buffers to improve the uniform distribution and stability of antibodies/antigens.

•Latex Agglutination Tests: Enhances the coating efficiency of latex particles for target antigens and reduces non-specific binding.

•PCR/qPCR: Acts as an additive in PCR reaction systems to suppress nucleic acid secondary structure formation and improve amplification efficiency.

•LAMP/CRISPR: Modulates reaction viscosity and stabilizes Cas proteins or primer-template complexes.

•Chip Lubrication: Reduces bubble formation and liquid retention in microchannels, increasing detection throughput.

•Test Strip Coating: Improves liquid diffusion speed on fibrous membranes, shortening detection time.

•Microplate Pre-treatment: Lowers surface tension, promotes the adsorption of enzyme-labeled antibodies, and reduces background noise.

•Immunoassays: 0.1%–1% (e.g., ELISA dilution/blocking buffers).

•Molecular Diagnostics: 0.5%–3% (e.g., PCR reaction systems).

•Microfluidic Chips: 0.01%–0.1% (as a lubricant).

•Sustained-Release Systems: 5%–10% (adjust based on the release rate of the target molecule).

•Heating Dissolution: Heat above 60°C to accelerate dissolution; viscosity properties are restored upon cooling.

•Gradient Addition: Avoid abrupt system changes by adding high concentrations of PEG 8000 gradually.

•Store sealed in a dry, light-protected environment (recommended 15–25°C) to prevent moisture absorption, caking, or high-temperature degradation.

•Slight stratification may occur during long-term storage; shake well before use.

•Temperature Sensitivity: High temperatures (>80°C) may cause molecular chain breakage or loss of solubilization capacity.

•pH Compatibility: Stable within pH 4–10; prone to hydrolysis and inactivation under strongly acidic/alkaline conditions.

•Plastic Containers: Prolonged contact may cause swelling of polyethylene (PE) or polypropylene (PP) containers; short-term use is recommended, or switch to glass/stainless steel containers.

•Protein Interference: Excess may adsorb to antibodies or antigens; optimize concentration through pre-experiments.

•Ionic Surfactants: Combination with strong ionic reagents (e.g., SDS) may cause precipitation.

•Hydrophobic Substances: Verify whether its solubilization effect on target molecules meets requirements.