•Enzyme/Protein Stabilizer: Encapsulates biomolecules such as glucose oxidase and peroxidase via its polymer chains, immobilizing them and preventing inactivation due to temperature, pH fluctuations, or mechanical shear.

•Protective Colloid: Inhibits the aggregation of nanoparticles (e.g., colloidal gold, quantum dots), maintaining the dispersion and signal stability of labeled probes.

•Film-Forming Ability: Forms a dense film on the surface of test strips or sensors, protecting active ingredients (e.g., enzymes, antibodies) from environmental interference (e.g., oxidation, humidity changes).

•Sustained-Release Carrier: Controls the release rate of reagents through physical embedding (e.g., gradual release of glucose oxidase in blood glucose test strips, extending the reaction window).

•Interferent Adsorption: Binds impurities such as phenols and tannins in samples, reducing interference with detection reactions (similar to the principle of plant enzyme purification).

•Chromatographic Separation Aid: Adsorbs aromatic acids in chromatographic media, improving the separation efficiency of target molecules.

•Enzyme Stabilizer: Used for the immobilization of glucose oxidase (GOD) or hexokinase (HK) to prevent enzyme inactivation (e.g., in the core layer of blood glucose test strips).

•Reaction Layer Film Formation: Forms a uniform film on the surface of test strips to ensure full contact between the enzyme and the sample and to control the reaction rate.

•Marker Protection: Stabilizes antibodies labeled with colloidal gold/fluorescent microspheres, preventing aggregation or precipitation during storage.

•Blocking Solution: Blocks nonspecific sites on nitrocellulose membranes, reducing background signals (particularly suitable for high-viscosity samples such as whole blood).

•Nucleic Acid Extraction: Adsorbs inhibitors such as polysaccharides and polyphenols in samples, improving the amplification efficiency of PCR/qPCR.

•CRISPR Detection: Stabilizes Cas protein-guide RNA complexes, enhancing the stability of gene editing reagents.

•Enzymatic Reaction Systems: Serves as a stabilizer for HRP (horseradish peroxidase) or ALP (alkaline phosphatase), extending the substrate color development time.

•Microfluidic Chips: Improves the wettability of hydrophobic channels, reducing liquid flow resistance.

Luvitec  is a trademark of BASF

•Enzyme Stabilizer: 0.5%–2% (impact on enzyme activity requires validation).

•Test Strip Film Formation: 1%–3% (dissolve in water or ethanol, then coat and dry).

•Blocking/Dilution Buffer: 0.2%–1% (e.g., 0.5%–1% is commonly used in ELISA blocking buffers).

•Interferent Adsorption: 2%–5% (mix with sample pretreatment solution, then centrifuge to remove precipitates).

•Direct Dissolution: Readily soluble in water and ethanol; slow stirring is required to avoid clumping (high molecular weight PVP K-90 requires longer dissolution time).

•Pre-mixed Storage: Prepare a 5%–10% stock solution; store refrigerated and avoid microbial contamination.

•Store sealed in a dry, cool place (recommended 15–25°C) to prevent moisture absorption, clumping, or microbial growth.

•Slight yellowing may occur during long-term storage but does not affect functionality (store protected from light).

•Temperature Sensitivity: High temperatures (>70°C) may cause molecular chain breakdown, losing film-forming ability.

•pH Compatibility: Stable within pH 3–10; may degrade under strongly acidic/alkaline conditions.

•Plastic Containers: Prolonged contact may swell polystyrene (PS) containers; use glass or stainless steel containers instead.

•Protein Interference: Excess may adsorb antigens/antibodies; optimize concentration through pre-experiments.

•Strong Oxidizers: May undergo oxidation reactions with high concentrations of ammonium persulfate, sodium hypochlorite, etc.

•Polyvalent Cations: May form gels or precipitates when combined with Ca²⁺, Mg²⁺, etc.