RZ (Purity Value/Reinheitszahl) is the ratio of absorbances A403/A275 measured in deionized water at a concentration of 0.5-1.0 mg/ml. It is a measure of heme content, not enzymatic activity. Preparations with a high RZ value may still exhibit low enzymatic activity.Unit DefinitionOne unit is defined as the amount of enzyme that produces 1.0 mg of purpurogallin from pyrogallol in 20 seconds at 20°C and pH 6.0.General DescriptionHorseradish peroxidase (HRP) is isolated from horseradish (Armoracia rusticana) and belongs to the ferriprotoporphyrin group of peroxidases. HRP is a single-chain polypeptide containing four disulfide bonds. It is a glycoprotein with a carbohydrate content of 18%. Depending on the specific isoenzyme, the carbohydrate composition includes galactose, arabinose, xylose, fucose, mannose, glucosamine, and galactosamine. Its molecular weight (~44 kDa) comprises the polypeptide chain (33,890 Da), hemin plus Ca²⁺ (~700 Da), and carbohydrates (~9,400 Da). There are at least seven HRP isoenzymes. The isoelectric points of horseradish peroxidase isoenzymes range from 3.0 to 9.0.
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