Product Features:

1. The QDC-2 quantum dots are spherical in shape with uniform morphology and contain a shell layer, making them more rigid. 

2. Stable performance, without fluorescence attenuation under various conditions of conventional medical, food, and drug testing samples. 

3. High fluorescence intensity, quantum yield over 90%, and strong anti-bleaching ability. 

4. High sensitivity, facilitating the development of femtoliter-level detection reagents. 

5. Uniform particle size, consistent among batches. 

6. It can directly replace and be compatible with the time-resolved platform. 

Physical properties: 

│Appearance: Orange-red 

│Chemical group: -COOH 

│Particle size: 120 ± 5 nm 

│Excitation wavelength: 340 - 390 nm 

│Emission wavelength: 610 - 620 nm 

│ Fluorescence conversion rate: ≥ 90% 

│Half-wavelength width: < 35 nm 

│Application: Fluorescence chromatography, EIA, FISH 

│Storage temperature: 4 - 37℃, 2 years, no freezing allowed

Coupling Procedure

1. Sequentially add 1 mL of the above buffer, quantum dot microspheres, and antibody into a 2 mL EP tube. Finally, add EDC, quickly cap the tube, and shake vigorously up and down or vortex for approximately 10 seconds. Incubate in a 37 °C shaker for 3 hours. If no precipitation is observed, proceed to the next step.

2. Place the EP tube in a refrigerated centrifuge and centrifuge at 5,000–15,000 rpm for 3–5 minutes. Discard the supernatant, and resuspend the pellet manually using coupling storage solution or by sonication at 60 W. Store at 4 °C for later use.

Coupling Storage Solution

The coupling storage solution is generally PBS buffer containing 1% BSA and certain protective agents (such as sucrose, trehalose, or proprietary process protectants selected by the manufacturer). It can be adjusted with reference to the original colloidal gold or fluorescence storage system; optimization is recommended. If severe resuspension failure occurs during centrifugation, first consider whether significant precipitation has formed during the coupling process. If no precipitation is present, adding an appropriate protective buffer prior to centrifugation may be considered.