ET SSB (Extreme Thermostable Single-Stranded DNA Binding Protein) is a single-stranded DNA-binding protein isolated from thermophilic microorganisms, with a molecular weight of 16 kDa . It remains fully active even after incubation at 95°C for 60 minutes . Owing to its extreme thermostability, ET SSB can be employed in various applications that require high-temperature conditions, such as nucleic acid amplification and sequencing .

Scope of Application:

Improve the processivity of DNA polymerase .

Stabilize and label ssDNA structures .

Increase the yield and specificity of PCR and isothermal amplification reactions .

Enhance the yield and efficiency of reverse transcription during RT-PCR .

Improve DNA sequencing in regions with strong secondary structures .

Enhance the activity of RecA in ssDNA binding and strand exchange .

For every 50 μl reaction system, add 50 - 200 ng of ET SSB.